Alternative to 2D gel electrophoresis – OFFGEL electrophoresis combined with high-sensitivity on-chip protein detection

Two-dimensional gel electrophoresis (2D-GE) employs isoelectric focusing in the first dimension and a separation of the proteins according to their molecular weights in the second dimension. The gels are then commonly stained using silver stain to visualize the protein pattern. This is a tedious and time-consuming procedure. Here, we demonstrate a combination of two newly developed methods – OFFGEL fractionation based on isoelectric point, and high-sensitivity on-chip electrophoresis with the Agilent 2100 bioanalyzer. This powerful combination performs an analytical 2D-GE-type analysis with excellent sensitivity and reproducibility. The ease of use is significantly improved compared with the traditional 2DGE method. Christian Wenz Andreas Rüfer Application Note